Mouse IGFBP-3 PicoKine ELISA Kit | Boster EK0387 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse IGFBP-3 PicoKine ELISA Kit

catalog :

EK0387

quantity :

96 wells/kit, with removable strips.

price :

380 USD

more info or order :

Boster product webpage

citations: 1

Reference
Shukla S, MacLennan G, Fu P, Gupta S. Apigenin attenuates insulin-like growth factor-I signaling in an autochthonous mouse prostate cancer model. Pharm Res. 2012;29:1506-17 pubmed publisher

image

image 1 :

Mouse IGFBP-3 PicoKine ELISA Kit standard curve

product information

sku :

EK0387

status :

Enabled

name :

Mouse IGFBP-3 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

IGFBP3

price :

380 USD

assay range :

156pg/ml-10,000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Mouse IGFBP3 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IGFBP3 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP-3. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

B3KPF0

host :

immunogen :

Expression system for standard: NSO; Immunogen sequence: G28Q-K291

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates and serum.

applications :

ELISA

reactivity :

Mouse

image labels :

Mouse IGFBP-3 PicoKine ELISA Kit standard curve

assay procedure step 1 :

10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml

background :

Insulin-like growth factor(IGF)-binding protein-3(IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene(IGFBP3) expression. Insulin-like growth factor-binding protein(IGFBP)-3, well characterized as the carrier of insulin-like growth factor(IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant mouse IGFBP-3, consisting of 270 amino acids with the molecular mass of 29.4kDa. As a result of glycosylation, the molecular mass is 40-50KDa.

competitor equivalent skus :

sc 6004 sc 9028 sc 374365 sc 6003 sc 365936 sc 130786 sc 135947

research category :

Associated Proteins, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Diabetes Associated, Growth Factors/Hormones, Metabolism, Obesity, Protein Phosphorylation, Receptors, Signal Transduction, Tyrosine Phosphatases

synonyms :

cDNA FLJ31712 fis, clone NT2RI2006445, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA FLJ34387 fis, clone HCHON1000191, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA FLJ38550 fis, clone HCHON2002123, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA PSEC0177 fis, clone OVARC1000287, highly similar to Insulin-like growth factor-binding protein 3 ;

gene full name :

insulin-like growth factor binding protein 3

molecular weight :

8708 MW

protein name :

Insulin-like growth factor-binding protein 3

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 269 1078 5499 266 1016 5861 Standard deviation 13.98 55.8%1 335.43 15.42 61.97 380.96 CV(%) 5.2% 5.4% 6.1% 5.8% 6.1% 6.5%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 269 246 224 238 244 16.31 6.6% Sample 2 1078 1247 1209 1042 1144 86.01 7.5% Sample 3 5499 5733 5821 5121 5543 270.83 4.8% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 156 312.5 625 125000 25000 5000 10000 O.D. 0.033 0.081 0.111 0.187 0.317 0.735 1.251 2.029

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

last modified :

3/7/19 6:53

more info or order :

Boster product webpage