Human IGFBP-3 PicoKine ELISA Kit | Boster EK0386 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IGFBP-3 PicoKine ELISA Kit

catalog :

EK0386

quantity :

96 wells/kit, with removable strips.

citations: 1

Reference
Duron E, Vidal J, Funalot B, Brunel N, Viollet C, Seux M, et al. Insulin-like growth factor I, insulin-like growth factor binding protein 3, and atrial fibrillation in the elderly. J Gerontol A Biol Sci Med Sci. 2014;69:1025-32 pubmed publisher

product information

sku :

EK0386

status :

Enabled

name :

Human IGFBP-3 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

IGFBP3

assay range :

156.2pg/ml-10000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human IGFBP3 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IGFBP3 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IGFBP-3. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

B3KPF0

host :

immunogen :

Expression system for standard: NSO; Immunogen sequence: A28Q-291

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human IGFBP-3 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml

background :

Insulin-like growth factor(IGF)-binding protein-3(IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene(IGFBP3) expression. Insulin-like growth factor-binding protein(IGFBP)-3, well characterized as the carrier of insulin-like growth factor(IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.

competitor equivalent skus :

sc 6004 sc 9028 sc 374365 sc 6003 sc 365936 sc 130786 sc 135947

research category :

Angiogenesis, Apoptosis, Associated Proteins, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Diabetes Associated, Growth Factors/Hormones, Metabolism, Obesity, Protein Phosphorylation, Receptors, Signal Transduction, Tyrosine Phosphatases

synonyms :

cDNA FLJ31712 fis, clone NT2RI2006445, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA FLJ34387 fis, clone HCHON1000191, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA FLJ38550 fis, clone HCHON2002123, highly similar to Insulin-like growth factor-binding protein 3 ;cDNA PSEC0177 fis, clone OVARC1000287, highly similar to Insulin-like growth factor-binding protein 3 ;

gene full name :

insulin-like growth factor binding protein 3

molecular weight :

8708 MW

protein name :

Insulin-like growth factor-binding protein 3

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 271 1607 5480 253 1639 5548 Standard deviation 16.53 69.1 350.72 19.73 95.06 437.8%9 CV(%) 6.1% 4.3% 6.4% 7.8% 5.8% 7.9%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 271 288 290 253 275 14.94 5.4% Sample 2 1607 1578 1461 1560 1551 54.87 3.5% Sample 3 5480 5896 5868 5913 5789 179.26 3% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.034 0.092 0.160 0.230 0.397 0.691 1.251 1.710

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53