Mouse IGFBP2 PicoKine ELISA Kit | Boster EK0385 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse IGFBP2 PicoKine ELISA Kit

catalog :

EK0385

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

image

image 1 :

Mouse IGFBP2 PicoKine ELISA Kit standard curve

product information

sku :

EK0385

status :

Enabled

name :

Mouse IGFBP2 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

IGFBP2

price :

369 USD

assay range :

125pg/ml-8000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Mouse IGFBP2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. IGFBP2 tissue specificity: Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP2. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P47877

host :

immunogen :

Expression system for standard: NSO; Immunogen sequence: E35-Q305

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Mouse

image labels :

Mouse IGFBP2 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml

background :

Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

competitor equivalent skus :

sc 6002 sc 13096 sc 365368 sc 6001 sc 25285 sc 130070 sc 515134

research category :

Cancer, Extracellular Matrix, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Signal Transduction

synonyms :

Insulin-like growth factor-binding protein 2;IBP-2;IGF-binding protein 2;IGFBP-2;mIGFBP-2;Igfbp2;Igfbp-2;

gene full name :

Insulin-like growth factor-binding protein 2

molecular weight :

32847 MW

protein function :

Inhibits IGF-mediated growth and developmental rates (By similarity). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

subcellular localization :

Secreted.

tissue specificity :

Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary.

protein name :

Insulin-like growth factor-binding protein 2

sequence similarities :

Contains 1 IGFBP N-terminal domain.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 160 970 3281 169 968 3467 Standard deviation 7.52 76.63 203.42 8.95 86.15 256.55 CV(%) 4.7% 7.9% 6.2% 5.3% 8.9% 7.4%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 160 171 174 173 169 5.59 3.3% Sample 2 970 1024 949 988 982 27.52 2.8% Sample 3 3281 3102 2889 3320 3148 170.63 5.4% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 125 250 500 1000 2000 4000 8000 O.D. 0.025 0.123 0.180 0.312 0.546 0.919 1.445 2.036

tmb incubation time :

15-25min

principle :

monoclonal antibody from rat polyclonal antibody from goat

last modified :

4/16/19 4:38

more info or order :

Boster product webpage