Human IGF-1 ELISA Kit PicoKine® | Boster EK0376 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human IGF-1 ELISA Kit PicoKine®

catalog :

EK0376

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0376

Product Name :

Human IGF-1 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human IGF-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human IGF1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Reactivity :

Human

Predicted Reactivity :

Bovine, Horse, Rabbit

Application :

ELISA

Gene Name :

IGF1

Uniprot ID :

P05019

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: G49-A118

Cross-reactivity :

There is no detectable cross-reactivity with IGF-2.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<50 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

insulin like growth factor 1

Synonyms :

Insulin-like growth factor I; IGF-I; Mechano growth factor; MGF; Somatomedin-C; IGF1; IBP1

Protein Name :

Insulin-like growth factor I

Molecular Weight :

21841 MW

Protein Function :

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1.

Subcellular Localization :

Secreted.

Sequence Similarities :

Belongs to the insulin family.

Background :

Insulin-like growth factor 1 (IGF-1), also called somatomedin C, is a protein that in humans is encoded by the IGF1 gene. It is mapped to 12q23.2. The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.