Human GM-CSF ELISA Kit PicoKine® | Boster EK0364 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human GM-CSF ELISA Kit PicoKine®

catalog :

EK0364

quantity :

96 wells/kit, with removable strips.

price :

400 USD

more info or order :

Boster product webpage

citations: 1

Reference
Amiri S, Ghazanfari T, Yaraee R, Salimi H, Ebtekar M, Shams J, et al. Serum levels of GM-CSF 20 years after sulfur mustard exposure: Sardasht-Iran Cohort Study. Int Immunopharmacol. 2009;9:1499-503 pubmed publisher

image

image 1 :

Human GM-CSF PicoKine ELISA Kit standard curve

product information

SKU :

EK0364

Product Name :

Human GM-CSF ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

400 USD

Description :

Human GM-CSF ELISA Kit PicoKine® (96 Tests). Quantitate Human CSF2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

CSF2

Uniprot ID :

P04141

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: A18-E144

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<1 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Granulocyte-macrophage colony-stimulating factor

Synonyms :

Granulocyte-macrophage colony-stimulating factor;GM-CSF;Colony-stimulating factor;CSF;Molgramostin;Sargramostim;CSF2;GMCSF;

Protein Name :

Granulocyte-macrophage colony-stimulating factor

Molecular Weight :

16295 MW

Protein Function :

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subcellular Localization :

Secreted.

Sequence Similarities :

Belongs to the GM-CSF family.

Background :

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.

Research Category :

Cytokines, Immunology, Innate Immunity

more info or order :

Boster product webpage