Human FLT3/FLK2 ELISA Kit PicoKine® | Boster EK0352 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human FLT3/FLK2 ELISA Kit PicoKine®

catalog :

EK0352

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0352

Product Name :

Human FLT3/FLK2 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human FLT3/FLK2 ELISA Kit PicoKine® (96 Tests). Quantitate Human FLT3 in cell culture supernatants, lysates, tissue, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

FLT3

Uniprot ID :

P36888

Immunogen :

Expression system for standard: NS0; Immunogen sequence: N27-N541

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<50 pg/ml

Sample Type :

cell culture supernatants, lysates, tissue, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

fms related tyrosine kinase 3

Synonyms :

Receptor-type tyrosine-protein kinase FLT3; FL cytokine receptor; Fetal liver kinase-2; FLK-2; Fms-like tyrosine kinase 3; FLT-3; Stem cell tyrosine kinase 1; STK-1; CD135; FLT3; CD135; FLK2; STK1

Protein Function :

Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.

Tissue Specificity :

Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells. Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia.

Background :

Cluster of differentiation antigen 135 (CD135) also known as fms like tyrosine kinase 3 (FLT-3), receptor-type tyrosine-protein kinase FLT3, or fetal liver kinase-2 (Flk2) is a protein that in humans is encoded by the FLT3 gene. It is mapped to 13q12.2. This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.