Mouse Fibronectin ELISA Kit PicoKine® | Boster EK0351 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse Fibronectin ELISA Kit PicoKine®

catalog :

EK0351

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0351

Product Name :

Mouse Fibronectin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse Fibronectin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

FN1

Uniprot ID :

P11276

Immunogen :

Expression system for standard: from plasma

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<15 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Capture/Detection Antibodies :

polyclonal antibody from rabbit, polyclonal antibody from rabbit

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Fibronectin

Synonyms :

Fibronectin; FN; Anastellin; Fn1

Protein Name :

Fibronectin

Protein Function :

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling (By similarity).

Subcellular Localization :

Secreted, extracellular space, extracellular matrix.

Tissue Specificity :

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.

Background :

Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.