Human FGF9 PicoKine ELISA Kit | Boster EK0348 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human FGF9 PicoKine ELISA Kit

catalog :

EK0348

quantity :

96 wells/kit, with removable strips.

product information

sku :

EK0348

status :

Enabled

name :

Human FGF9 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

FGF9

assay range :

62.5pg/ml-4000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human FGF9 with <15pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. FGF9 tissue specificity: Glial cells.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FGF9. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P31371

host :

immunogen :

Expression system for standard: sf21; Immunogen sequence: M1-S208

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<15pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

predicted reactivity :

Bovine, Horse, Rabbit

image labels :

Human FGF9 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml

background :

Fibroblast growth factor-9(FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells. The human FGF-9 cDNA cloned by using oligonucleotide probes encodes a polypeptide consisting of 208 amino acids. Sequence similarity to other members of the FGF family has been estimated to be around 30%. FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma. FGF9 is produced and secreted by the prostatic stromal cells. It is a potent mitogen for both prostatic epithelial and stromal cells in culture. FGF9 is an abundant secreted growth factor that can act as both a paracrine mitogen for epithelial cells and an autocrine mitogen for stromal cells. Overexpression of this paracrine and autocrine growth factor may play an important role in the epithelial and stromal proliferation in benign prostatic hyperplasia.4 As a result of glycosylation, the molecular mass is 25-27KDa.

research category :

Angiogenesis, Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Neurogenesis, Neurology Process, Neuroscience, Reproduction, Signal Transduction

synonyms :

Fibroblast growth factor 9;FGF-9;Glia-activating factor;GAF;Heparin-binding growth factor 9;HBGF-9;FGF9;

gene full name :

Fibroblast growth factor 9

molecular weight :

23441 MW

protein function :

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. May have a role in glial cell growth and differentiation during development, gliosis during repair and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and growth stimulation of glial tumors.

subcellular localization :

Secreted.

tissue specificity :

Glial cells

protein name :

Fibroblast growth factor 9

sequence similarities :

Belongs to the heparin-binding growth factors family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 112 506 1252 115 527 1200 Standard deviation 6.72 30.86 88.89 7.13 35.83 96 CV(%) 6% 6.1% 7.1% 6.2% 6.8% 8%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 101 99 105 104 4.96 4.7% Sample 2 506 485 519 502 503 12.14 2.4% Sample 3 1252 1335 1172 1129 1222 78.76 6.4% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 4000 2000 4000 2000 4000 2000 4000 O.D. 0.087 0.097 0.115 0.145 0.247 0.500 1.026 2.188

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53