Human FGF4 PicoKine ELISA Kit | Boster EK0345 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human FGF4 PicoKine ELISA Kit

catalog :

EK0345

quantity :

96 wells/kit, with removable strips.

product information

sku :

EK0345

status :

Enabled

name :

Human FGF4 PicoKine ELISA Kit

category name :

Picokine ELISA Kits, Cytokine Network

gene name :

FGF4

assay range :

62.5pg/ml-4000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human FGF4 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. FGF4 tissue specificity:

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FGF4. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P08620

host :

immunogen :

Expression system for standard: E.coli; Immunogen sequence: A31-L206

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<10pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human FGF4 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml

background :

Fibroblast growth factor 4 is a protein that in humans is encoded by the FGF4 gene. The protein encoded by this gene is a member of the fibroblast growth factor(FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity. This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. FGF4, a direct target of LEF1 and Wnt signaling, can rescue the arrest of tooth organogenesis in Lef1(-/-) mice.

research category :

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Oncoproteins, Oncoproteins/Suppressors, Signal Transduction

synonyms :

Fibroblast growth factor 4;FGF-4;Heparin secretory-transforming protein 1;HST;HST-1;HSTF-1;Heparin-binding growth factor 4;HBGF-4;Transforming protein KS3;FGF4;HST, HSTF1, KS3;

gene full name :

Fibroblast growth factor 4

molecular weight :

22048 MW

protein function :

Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis.

subcellular localization :

Secreted .

protein name :

Fibroblast growth factor 4

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 132 529 1434 135 579 1324 Standard deviation 9.76 32.26 83.17 12.01 45.74 101.94 CV(%) 7.4% 6.1% 5.8% 8.9% 7.9% 7.7%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 132 145 143 124 136 8.51 6.2% Sample 2 529 557 529 611 556 33.47 6% Sample 3 1434 1303 1232 1245 1303 79.94 6.1% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 4000 2000 4000 2000 4000 2000 4000 O.D. 0.053 0.078 0.109 0.153 0.271 0.536 0.824 1.496

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

3/7/19 6:53