Human Erythropoietin/EPO ELISA Kit PicoKine® | Boster EK0332 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Erythropoietin/EPO ELISA Kit PicoKine®

catalog :

EK0332

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK0332

Product Name :

Human Erythropoietin/EPO ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human Erythropoietin/EPO ELISA Kit PicoKine® (96 Tests). Quantitate Human EPO in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 1mIU/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

EPO

Uniprot ID :

P01588

Immunogen :

Expression system for standard: CHO; Immunogen sequence: A28-R193

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

3.12 mIU/ml - 200 mIU/ml

Sensitivity :

<1mIU/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

erythropoietin

Synonyms :

Erythropoietin; Epoetin; EPO

Protein Function :

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Subcellular Localization :

Secreted.

Tissue Specificity :

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Background :

Erythropoietin (EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. It is mapped to 7q22.1. This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.