Human EGF ELISA Kit PicoKine® | Boster EK0325 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human EGF ELISA Kit PicoKine®

catalog :

EK0325

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 3

Reference
Scalabrino G, Veber D, De Giuseppe R, Roncaroli F. Low levels of cobalamin, epidermal growth factor, and normal prions in multiple sclerosis spinal cord. Neuroscience. 2015;298:293-301 pubmed publisher
Azuma N, Katada Y, Kitano S, Sekiguchi M, Kitano M, Nishioka A, et al. Rapid decrease in salivary epidermal growth factor levels in patients with Sjögren's syndrome: A 3-year follow-up study. Mod Rheumatol. 2015;25:876-82 pubmed publisher
Nojiri T, Yoshizato T, Fukami T, Obama H, Yagi H, Yotsumoto F, et al. Clinical significance of amphiregulin and epidermal growth factor in colostrum. Arch Gynecol Obstet. 2012;286:643-7 pubmed publisher

image

image 1 :

Human EGF PicoKine ELISA Kit standard curve

product information

SKU :

EK0325

Product Name :

Human EGF ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Human EGF ELISA Kit PicoKine® (96 Tests). Quantitate Human EGF in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

EGF

Uniprot ID :

E7EVD2

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: N971-R1023

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

4.7 pg/ml - 300 pg/ml

Sensitivity :

<1 pg/ml

Sample Type :

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Pro-epidermal growth factor

Synonyms :

Pro-epidermal growth factor;EGF;Epidermal growth factor;Urogastrone;EGF;

Protein Name :

Pro-epidermal growth factor

Molecular Weight :

133994 MW

Protein Function :

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

Tissue Specificity :

Expressed in kidney, salivary gland, cerebrum and prostate.

Sequence Similarities :

Contains 9 EGF-like domains.

Background :

Epidermal growth factor or EGF is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF acts by binding with high affinity to epidermal growth factor receptor (EGFR) on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. EGF results in cellular proliferation, differentiation, and survival. It also has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. EGF has strong expression in kidney, salivary gland, cerebrum, and prostate, moderate expression in trachea and thyroid, and low expression in bone marrow, heart, spleen, thymus, uterus, and colon. No expression was detected in adrenal gland, liver, lung, cerebellum, placenta, and small intestine.

Research Category :

Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Growth Factors, Growth Factors/Hormones, Signal Transduction

more info or order :

Boster product webpage