Human Angiogenin/ANG ELISA Kit PicoKine® | Boster EK0305 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Angiogenin/ANG ELISA Kit PicoKine®

catalog :

EK0305

quantity :

96 wells/kit, with removable strips.

price :

400 USD

more info or order :

Boster product webpage

citations: 2

Reference
Matter R, Abdelmaksoud A, Shams M, Bebawy E. Serum angiogenin level in sickle cell disease and beta thalassemia patients. Pediatr Hematol Oncol. 2014;31:50-6 pubmed publisher
Yalcindag A, Gedik Oğuz Y, Yalçindağ F. The relationship between serum levels of angiogenin, bFGF, VEGF, and ocular involvement in patients with Behçet's disease. Graefes Arch Clin Exp Ophthalmol. 2013;251:1807-12 pubmed publisher

product information

SKU :

EK0305

Product Name :

Human Angiogenin/ANG ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

400 USD

Description :

Human Angiogenin/ANG ELISA Kit PicoKine® (96 Tests). Quantitate Human ANG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

ANG

Uniprot ID :

P03950

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: Q25-P147

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

78 pg/ml - 5,000 pg/ml

Sensitivity :

<12 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Angiogenin

Synonyms :

Angiogenin;3.1.27.-;Ribonuclease 5;RNase 5;ANG;RNASE5;

Protein Name :

Angiogenin

Molecular Weight :

16550 MW

Protein Function :

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.

Subcellular Localization :

Nucleus . Secreted, extracellular space, extracellular matrix, basement membrane. Nucleus, nucleolus. Rapidly endocytosed by target cells and translocated to the nucleus where it accumulates in the nucleolus and binds to DNA.

Tissue Specificity :

Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons.

Sequence Similarities :

Belongs to the pancreatic ribonuclease family.

Background :

Angiogenin (Ang) also known as ribonuclease 5 is a protein that in humans is encoded by the ANG gene. Angiogenin is a potent stimulator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. Hooper et al. (2003) reviewed the evidence that angiogenins are involved in host defense and noted that inflammation provokes upregulated ANG mRNA expression in liver and an increase in detectable ANG protein in serum. Weremowicz et al. (1989, 1990) assigned the human angiogenin gene to chromosome 14q11.

Research Category :

RNA Processing, Signal Transduction, Translation

more info or order :

Boster product webpage