antibody

Anti-ER alpha Antibody (Monoclonal)

CI1131

100 ul

380 USD

monoclonal

mouse

nonconjugated

western blot, ELISA, immunoprecipitation, chromatin immunoprecipitation, ChIP-Seq

Anti-ER alpha Antibody (Monoclonal)

380 USD

This antibody is raised in mouse against human ERalpha (estrogen receptor alpha), using a synthetic peptide.

Liquid

Mouse

Ascites fluid from mouse containing 0.05% azide.

Ascites fluid

Monoclonal

Mouse monoclonal antibody for human ERalpha (estrogen receptor alpha). Tested with ChIP,ChIP-seq,ELISA,WB,GS,IHC,IP, in Human, Mouse.

100 ul

Human; Mouse

ChIP; ChIP-qPCR; ChIP-seq; ELISA; IP; WB

The estrogen receptor alpha (ERalpha, UniProt/Swiss-Prot entry P03372) belongs to the family of nuclear hormone receptors, which are ligand-activated transcription factors. They are important for the regulation of gene expression, cellular proliferation and differentiation, sexual development and reproductive function. Estrogen receptors are also involved in pathological processes such as breast cancer, and osteoporosis. ERalpha can regulate transcription by direct binding to estrogen response elements (EREs) in the DNA or by interaction with other transcription factors. It may also form a heterodimer with ERbeta.

Estrogen receptor

ESR1

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA- binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF- kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA- binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.

Estrogen receptor

signal transduction signaling pathway nuclear signaling nuclear hormone receptors estrogen neuroscience endocrine system gonadotrophic axis epigenetics and nuclear signaling nuclear signaling pathways nuclear receptors cancer chip'ing antibodies

Belongs to the nuclear hormone receptor family. NR3 subfamily.

At -20 for one year, at 4 for one month. Avoid repeated freezing and thawing.

Isoform 1: Nucleus . Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . A minor fraction is associated with the inner membrane.

Estrogen receptor;ER;ER-alpha;Estradiol receptor;Nuclear receptor subfamily 3 group A member 1;ESR1;ESR, NR3A1;

Widely expressed. Isoform 3 is not expressed in the pituitary gland.

P03372