Anti-p53 TP53 Antibody | Boster CI1060 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-p53 TP53 Antibody

catalog :

CI1060

quantity :

50µg/50µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human

application :

western blot, immunohistochemistry, flow cytometry, chromatin immunoprecipitation, ChIP-Seq

product information

SKU :

CI1060

Product Name :

Anti-p53 TP53 Antibody

Size :

50µg/50µl

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

WB, IHC, Flow Cytometry, ChIP-seq, ChIP-qPCR, ChIP

Application Details :

User need to optimize the dilution ratio for this antibody

Description :

Boster Bio Anti-p53 TP53 Antibody catalog # CI1060. Tested in ChIP, ChIP-qPCR, ChIP-seq, Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

TP53

Uniprot ID :

P04637

Immunogen :

This antibody is raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.

Form :

Liquid

Contents :

Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.

Purification :

Affinity purified

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

Cellular tumor antigen p53

Synonyms :

Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; TP53; P53;

Protein Name :

Cellular tumor antigen p53

Molecular Weight :

43653 MW

Protein Function :

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1- mediated transcriptional activation of PER2 (PubMed:24051492).

Subcellular Localization :

Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2. Translocates to mitochondria upon oxidative stress.

Tissue Specificity :

Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Boster recommends high sensitivity ChIP-seq Kit (CK1001 & CK1002) for Chromatin Immunoprecipitation.

Sequence Similarities :

Belongs to the p53 family.

Background :

The transcription factor p53 is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.

Research Category :

Apoptosis, Cell Biology, Intracellular, P53 Pathway