secondary antibody

Mouse Anti-Human IgG (H+L) Secondary Antibody, FITC Conjugate

BM2003

0.5 ml

Enabled

Mouse Anti-Human IgG (H+L) Secondary Antibody, FITC Conjugate

Secondary Antibodies, IHC ICC IF Antibodies

0.5ml / $130 1ml / $250

Polyclonal

1mg/ml

FITC

FITC Conjugated Mouse Anti-human IgG (H+L) secondary antibody This FITC conjugated antibody is specific for human IgG and shows no cross-reactivity with rabbit/goat/bovine IgG.

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all mouse serum proteins, except the specific antibody for human IgG.

Product Overview Product Name Mouse Anti-Human IgG (H+L) Secondary Antibody, FITC Conjugate Synonyms FITC-conjugated Mouse Anti-Human IgG; Mouse Anti-Human IgG-FITC Secondary Antibody; Fluorescein-labeled Mouse Anti-Human IgG Secondary Antibody Description Mouse Anti-Human IgG (H+L) Secondary Antibody, FITC Conjugate, for detection, localization and quantification of target proteins in a sample via indirect immunofluorescence in IHC-P, IHC-F, ICC, or FCM. Reagent Type Fluorophore-conjugated secondary antibody Conjugate FITC Host Mouse Target Species Human Antibody Class IgG Clonality Polyclonal Immunogen Whole molecule human IgG Purification Immunoaffinity chromatography Specificity Human IgG specific; No cross-reactivity with rabbit/goat/bovine IgG Form Supplied Liquid, concentrated buffered stock solution Formulation 0.5 mg HRP-conjugated secondary antibody . 0.01 M PBS (PH 7.4) . 0.01% Thimerosal . 50% glycerol Pack Size 0.5 ml Concentration 1 mg/ml Application Flow Cytometry (FCM), Immunohistochemistry (paraffin-embedded (IHC-P) and frozen(IHC-F) sections), Immunocytochemistry (ICC). *Our Boster Guarantee covers the use of this product in the above marked tested applications. Storage 4C for 1 year Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE Assay Information Sample Type Human primary-antibody-probed Single cell suspension, Formalin-fixed paraffin-embedded (FFPE) tissue sections, Thawed frozen samples (IHC-F) Assay Type Immunoanalytical Assay Purpose Protein detection/quantification Technique Immunofluorescence Equipment Needed Excitation light source; Filter set and detector: fluorescence microscope (can be combined with confocal microscope), fluorescence plate-reader, flow cytometer, or cell sorter Main Advantages Specific High signal-to-noise ratio High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Multiple FITC molecules bind to a single secondary antibody Fast Fewer number of processing steps - no need for adding a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; Fluorescence can be observed directly Quantifieable Allows quantification of detected signal Easy to Use Supplied in a workable liquid format Multiplex Compatibility Colocalization studies (multiple antigens concurrent detection), even in close proximity: using primary antibodies from different host species for simultaneous detection by fluorophore-conjugated secondary antibodies;using multiple differently colored fluorophores in the same experiment for targets differentiation Background . Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application. . Immunofluorescence is a technique used for light microscopy with a fluorescence microscope which utilizes fluorescent dyes as reporters. It is being employed in a variety of applications such as cellular imaging and flow cytometry and is commonly used to visualize the distribution of target molecules through a sample, to detect protein location and activation, to identify protein complex formation and conformational changes, to monitor biological processes in vivo. Fluorescent dyes (also known as fluorochromes, fluorophores, or simply fluors) are molecules that can absorb light of a specific energy and wavelength, thereby undergoing excitation, and then re-emit it at a lower energy and longer wavelength upon returning to the ground state.

0.5 ml

No

Flow Cytometry, IHC, ICC

Human

Boster Kit Box

2/11/19 19:28