secondary antibody

Rabbit Anti-Human IgG (H+L) Secondary Antibody, FITC Conjugate

BA1114

0.5 ml

Enabled

Rabbit Anti-Human IgG (H+L) Secondary Antibody, FITC Conjugate

Secondary Antibodies, IHC ICC IF Antibodies

0.5ml / $80 1ml / $150

Polyclonal

1mg/ml

FITC

FITC Conjugated Rabbit Anti-human IgG (H+L) secondary antibody This FITC conjugated antibody is specific for human IgG and shows no cross-reactivity with rat/mouse/goat/rabbit IgG.

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all rabbit serum proteins, except the specific antibody for human IgG.

Product Overview Product Name Rabbit Anti-Human IgG (H+L) Secondary Antibody, FITC Conjugate Synonyms FITC-conjugated Rabbit Anti-Human IgG; Rabbit Anti-Human IgG-FITC Secondary Antibody; Fluorescein-labeled Rabbit Anti-Human IgG Secondary Antibody Description Rabbit Anti-Human IgG (H+L) Secondary Antibody, FITC Conjugate, for detection, localization and quantification of target proteins in a sample via indirect immunofluorescence in IHC-P, IHC-F, ICC, or FCM. Reagent Type Fluorophore-conjugated secondary antibody Label FITC Host Rabbit Target Species Human Antibody Class IgG Clonality Polyclonal Immunogen Whole molecule human IgG Purification Immunoaffinity chromatography Specificity Human IgG specific Form Supplied Liquid, concentrated buffered stock solution Formulation 0.5 mg FITC-conjugated secondary antibody . 0.01 M PBS (PH 7.4) . 0.01% Thimerosal . 50% glycerol Pack Size 0.5 ml Concentration 1 mg/ml Application Flow Cytometry (FCM), Immunohistochemistry (paraffin-embedded (IHC-P) and frozen(IHC-F) sections), Immunocytochemistry (ICC) . *Our Boster Guarantee covers the use of this product in the above marked tested applications. Storage 4C for 1 year Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE Assay Information Sample Type Human primary-antibody-probed Single cell suspension, Formalin-fixed paraffin-embedded (FFPE) tissue sections, Thawed frozen samples (IHC-F) Assay Type Immunoanalytical Assay Purpose Protein detection/quantification Technique Immunofluorescence Equipment Needed Excitation light source; Filter set and detector: fluorescence microscope (can be combined with confocal microscope), fluorescence plate-reader, flow cytometer, or cell sorter Additional Materials Required Primary antibody against target antigen raised in human; Diluent Buffer (PBS or TBS); Application-specific reagents and appliances; Main Advantages Specific High signal-to-noise ratio High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody; Multiple FITC molecules bind to a single secondary antibody Fast Fewer processing steps - no need to add a substrate; Less optimization required compared to enzymatic detection; Generates strong signals in a relatively short time span; Fluorescence can be observed directly Quantifieable Allows quantification of detected signal Easy to Use Supplied in a workable liquid format Multiplex Compatibility Colocalization studies possible, even in close proximity: use primary antibodies from different host species for simultaneous detection by fluorophore-conjugated secondary antibodies; use multiple differently colored fluorophores in the same experiment for target differentiation Dynamic range Good linearity within detection limits Background . Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application. . Immunofluorescence is a technique used for light microscopy with a fluorescence microscope which utilizes fluorescent dyes as reporters. It is being employed in a variety of applications such as cellular imaging and flow cytometry and is commonly used to visualize the distribution of target molecules through a sample, to detect protein location and activation, to identify protein complex formation and conformational changes, and to monitor biological processes in vivo. Fluorescent dyes (also known as fluorochromes, fluorophores, or simply fluors) are molecules that can absorb light of a specific energy and wavelength, thereby undergoing excitation, and then re-emit it at a lower energy and longer wavelength upon returning to the ground state. . Fluorescent reporters widely used in biological research are of two types: organic compounds with a low molecular weight (0.2-1 kDa) typically containing numerous aromatic groups or plane or cyclic moieties with π bonds (e.g.FITC, TRITC, Alexa Fluor Dyes, DyLight Fluors), and biological fluorophores (e.g.green fluorescent protein (GFP), R-Phycoerythrin). FITC (Fluorescein isothiocyanate) is derivative of fluorescein where a hydrogen atom on the bottom ring of the structure is replaced by isothiocyanate functional group (-N=C=S), making it more reactive to amine and sulfhydryl groups on proteins. The excitation and emission wavelengths of FITC are 495 nm and 525 nm respectively. Like most fluorochromes, it is prone to photobleaching, i.e. losing fluorescing properties due to molecule structure degradation. Loss of activity caused by photobleaching can be controlled by reducing the intensity or time-span of light exposure, by increasing the concentration of the fluorophore, or by employing more robust fluorophores that are less prone to bleaching (e.g., Alexa Fluors, Seta Fluors, or DyLight Fluors). Analogs of FITC with greater photostability and higher fluorescence intensity tailored in various biological applications are Alexa 488 and DyLight 488.

0.5 ml

Rabbit

Human IgG (whole molecule).

At 4 C for one year.

Flow Cytometry, IHC, ICC

Human

Boster Kit Box

2/11/19 19:27