secondary antibody

Goat Anti-Human IgM Secondary Antibody, HRP Conjugate

BA1077

0.25 ml

Enabled

Goat Anti-Human IgM Secondary Antibody, HRP Conjugate

Secondary Antibodies

0.25mg, 0.5mg/ml / $80 0.5mg, 0.5mg/ml / $150

Polyclonal

1mg/ml

HRP

HRP Conjugated Goat Anti-human IgM (u-chain specific) secondary antibody This HRP conjugated antibody is specific for human IgM and shows no cross-reactivity with human IgA/IgG.

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for human IgM.

Product Overview Product Name Goat Anti-Human IgM Secondary Antibody, HRP Conjugate Synonyms HRP-conjugated goat anti-human IgM Description Goat Anti-Human IgM Secondary Antibody, HRP Conjugate, for the indirect sensitive immunodetection and/or quantification of target proteins through Dotblot, WB, or ELISA by assaying an HRP-catalyzed reaction product in the vicinity of the antigen-primary antibody-secondary antibody-HRP complex. Reagent Type Secondary antibody, reporter enzyme labeled Label HRP (Horseradish Peroxidase) Host Goat Target Species Human Antibody Class IgG Clonality Polyclonal Immunogen Whole molecule human IgM Purification Immunoaffinity Chromatography Specificity No cross-reactivity with human IgA/IgG- Form Supplied Liquid: concentrated buffered stock solution Formulation 0.25 mg HRP-conjugated secondary antibody . 0.01 M PBS (pH 7.4) . 50% glycerol Pack Size 0.25 ml Concentration 1 mg/ml Storage 4C for 1 year Application ELISA*, WB*, Dot blot . *Our Boster Guarantee covers the use of this product in the above marked tested applications. Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE Assay Information Sample Type SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates Assay Type Immunoassay Assay Purpose Protein detection/quantification Technique Indirect immunodetection of target antibody with HRP reporter enzyme Equipment Needed WB/Dot blot/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer Compatibility with Reagents Incompatible with sodium azide and metals . incompatible with high phosphate concentrations Main Advantages Specific High signal-to-noise ratio Sensitive Detects low-abundant targets due to an optimal number of HRP molecules per antibody High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC Fast Generates strong signals in a relatively short time span Quantifieable Allows quantification of detected signal Easy to Use Supplied in a workable liquid format Flexible HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates; Convenient HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes Background . Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest. Purified secondary antibodies are further solid phase adsorbed with other species serum proteins to minimize cross-reactivity in tissue or cell preparations, and are then modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application. . Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.

0.25 ml

Goat

Human IgM (whole molecule).

At 4 C for one year.

ELISA, WB

Human

Boster Kit Box

Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

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