secondary antibody

Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate

BA1054

0.5 ml

Enabled

Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate

Secondary Antibodies

0.5mg (free with primary ab) / $95 1mg ($85 with primary ab) / $180

Polyclonal

1mg/ml

HRP

HRP Conjugated Goat Anti-rabbit IgG (H+L) secondary antibody This HRP conjugated antibody is specific for rabbit IgG and shows no cross-reactivity with mouse/bovine IgG.

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for rabbit IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations.

Product Overview Product Name Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate Synonyms HRP-conjugated goat anti-rabbit IgG Description Goat Anti-Rabbit IgG (H+L) Secondary Antibody, HRP Conjugate, for the indirect sensitive immunodetection and/or quantification of target proteins through Dot Blot, WB, or ELISA by assaying an HRP-catalyzed reaction product in the vicinity of the antigen-primary antibody-secondary antibody-HRP complex. Reagent Type Secondary antibody, reporter enzyme labeled Label HRP (Horseradish Peroxidase) Host Goat Target Species Rabbit Antibody Class IgG Clonality Polyclonal Immunogen Whole molecule rabbit IgG Purification Immunoaffinity Chromatography Specificity Rabbit IgG specific; No cross-reactivity with related species Form Supplied Liquid: concentrated buffered stock solution Formulation 0.5 mg HRP-conjugated secondary antibody . 0.01 M PBS (PH 7.4) . 50% glycerol Pack Size 0.5 ml Concentration 1 mg/ml Application ELISA*, WB*, Dot blot . *Our Boster Guarantee covers the use of this product in the above marked tested applications. Storage 4C for 1 year Precautions FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR CLINICAL USE Assay Information Sample Type SDS-PAGE separated-, membrane-immobilized-, mouse primary-antibody-probed proteins from cell/tissue lysates Assay Type Immunoassay Assay Purpose Protein detection/quantification Technique Immunodetection of target antibody with HRP reporter enzyme Equipment Needed WB/Dot blot/ELISA instrumentation; X-ray film cassette or a charge-coupled device (CCD) imager; Spectrophotometer Compatibility with Reagents Incompatible with sodium azide and metals . incompatible with high phosphate concentrations Main Advantages Specific High signal-to-noise ratio Sensitive Detects low-abundant targets due to an optimal number of HRP molecules per antibody High Signal Amplification Multiple secondary antibodies can bind to a single primary antibody;Secondary antibodies Fc regions provide further binding locations for biotin, or enable the use of ABC and SABC Fast Generates strong signals in a relatively short time span Quantifieable Allows quantification of detected signal Easy to Use Supplied in a workable liquid format Flexible HRP: compatible with chromogenic, fluorogenic and chemiluminescent substrates; Convenient HRP’s small size: no interference with the primary/secondary antibody interaction; no steric hindrance to antibody/antigen complexes Background . Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. The host antiserum is then purified through immunoaffinity chromatography to remove all host serum proteins, except the specific antibody of interest, and are then modified with antibody fragmentation, label conjugation, etc. Secondary antibodies can be conjugated to a large number of labels, including enzymes, biotin, and fluorescent dyes/proteins. Here, the antibody provides the specificity to locate the protein of interest, and the label generates a detectable signal. The label of choice depends upon the experimental application. . Horseradish peroxidase (HRP) is extensively used for labeling secondary antibodies in ELISA, western blot, dot blot and immunohistochemistry. The HRP enzyme is made visible using a substrate that, when oxidized by HRP in the presence of hydrogen peroxide as an oxidizing agent, yields a characteristic change that is detectable by specific detection methods. The substrates commonly used with HRP fall into different categories including chromogenic, fluorogenic, and chemiluminescent substrates depending on whether they produce a colored, fluorimetric or luminescent derivative respectively. The intensity of the signal is proportional to peroxidase activity and is a measure of the number of enzyme molecules reacting, hence of the amount of recognized primary antibodies, and thus of the amount of target antigen.

0.5 ml

Goat

Rabbit IgG (whole molecule).

At 4 C for one year.

ELISA, WB

Rabbit

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