Anti-Chk1 Antibody | Boster A30437 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Chk1 Antibody

catalog :

A30437

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunocytochemistry

product information

sku :

A30437

status :

Enabled

name :

Anti-Chk1 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

CHEK1

price various sizes :

100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

description :

Polyclonal antibody for Chk1/CHEK1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF. Reactive species: Human. Chk1/CHEK1 information: Molecular Weight: 54434 MW; Subcellular Localization: Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B; Tissue Specificity: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.

short description :

Anti-Chk1 Antibody

size :

100 ug/vial

uniprot id :

O14757

host :

Rabbit

immunogen :

A peptide derived from human Chk1. Immunogen sequence location: 271-320

form :

Liquid

purification :

The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

application details :

User need to optimize the dilution ratio for this antibody

applications :

reactivity :

Human, Mouse, Rat

image labels :

Immunofluorescence analysis of A549 cells, using Chk1 Antibody. The picture on the right is blocked with the synthesized peptide.

research category :

Kinases/Phosphatases

synonyms :

Serine/threonine-protein kinase Chk1;2.7.11.1;CHK1 checkpoint homolog;Cell cycle checkpoint kinase;Checkpoint kinase-1;CHEK1;CHK1;

gene full name :

Serine/threonine-protein kinase Chk1

molecular weight :

54434 MW

protein function :

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser- 124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA. May regulate the transcription of genes that regulate cell- cycle progression through the phosphorylation of histones. Phosphorylates histone H3.1 (to form H3T11ph), which leads to epigenetic inhibition of a subset of genes. May also phosphorylate RB1 to promote its interaction with the E2F family of transcription factors and subsequent cell cycle arrest.

subcellular localization :

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.

tissue specificity :

Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.

last modified :

2/6/19 22:57