Anti-Sca1/Ly6A/E Antibody Picoband™ | Boster A30403 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Sca1/Ly6A/E Antibody Picoband™

catalog :

A30403

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

product information

SKU :

A30403

Product Name :

Anti-Sca1/Ly6A/E Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Mouse

Application(s) :

Flow Cytometry, IHC-P, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Mouse. Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Mouse. Flow Cytometry, 1-3μg/1x10^6 cells, Mouse.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Sca1/Ly6A/E Antibody Picoband™ catalog # A30403. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Mouse.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

Ly6a

Uniprot ID :

P05533

Immunogen :

A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E (48-75aa YPDGVCVTQEAAVIVDSQTRKVKNNLCL).

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Lymphocyte antigen 6A-2/6E-1

Synonyms :

Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1; SCA-1; T-cell-activating protein; TAP; Ly6a; Ly6;

Protein Name :

Lymphocyte antigen 6A-2/6E-1

Molecular Weight :

14377 MW

Protein Function :

T-cell activation.

Subcellular Localization :

Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity :

Widely expressed.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.