Anti-LST8/GBL Antibody | Boster A05357 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-LST8/GBL Antibody

catalog :

A05357

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

sku :

A05357

status :

Enabled

name :

Anti-LST8/GBL Antibody

category name :

Primary Antibodies, Polyclonal Antibodies

gene name :

MLST8

price various sizes :

25ug sample size / $99 25ug sample size / $99 100 ug / $299 100 ug / $299 100 ug+Free HRP Secondary BA1054 / $299 100 ug+Free Biotin Secondary BA1054 / $299 100 ug+Free Biotin Secondary BA1003 / $299 100 ug+Free Biotin Secondary BA1003 / $299

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide.

description :

Polyclonal antibody for GBL/MLST8 detection. Host: Rabbit.Size: 100 ug. Tested applications: WB. Reactive species: Human. GBL/MLST8 information: Molecular Weight: 35876 MW; Subcellular Localization: Cytoplasm ; Tissue Specificity: Broadly expressed, with highest levels in skeletal muscle, heart and kidney.

short description :

Rabbit IgG Polyclonal antibody for MLST8 detection. Tested with WB in Human.

size :

100 ug

sample size available :

25ug for $99, contact us for details

uniprot id :

Q9BVC4

host :

Rabbit

immunogen :

A partial length recombinant LST8/GBL protein (amino acids 100-320).

form :

Liquid

storage :

Store the antibody at 4 C, stable for 6 months. For long-term storage, store at -20 C. Avoid repeated freeze and thaw cycles.

application details :

Western blot analysis, 1-3 ug/ml.

applications :

reactivity :

Human

image labels :

Western blot analysis of LST8/GBL. Anti-LST8/GBL was used at 1 ug/ml on Kidney lysate.

competitor equivalent skus :

sc 86807 sc 86806

synonyms :

Target of rapamycin complex subunit LST8;TORC subunit LST8;G protein beta subunit-like;Gable;Protein GbetaL;Mammalian lethal with SEC13 protein 8;mLST8;MLST8;GBL, LST8;

gene full name :

Target of rapamycin complex subunit LST8

molecular weight :

35876 MW

protein function :

Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient- poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum- induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

subcellular localization :

cytoplasm

tissue specificity :

Broadly expressed, with highest levels in skeletal muscle, heart and kidney.

protein name :

Target of rapamycin complex subunit LST8

last modified :

2/6/19 22:57