Anti-TRAIL-R4 Antibody | Boster A05136 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-TRAIL-R4 Antibody

catalog :

A05136

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry

product information

sku :

A05136

status :

Enabled

name :

Anti-TRAIL-R4 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

TNFRSF10D

price various sizes :

100ug / $363 100ug+Free HRP Secondary BA1054 / $363 100ug+Free Biotin Secondary BA1003 / $363

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Liquid. In PBS containing 0.02% sodium azide.

description :

Rabbit Polyclonal antibody for TNFRSF10D detection. Tested positive for IF, IHC, WB in Human, Mouse, Rat

short description :

Rabbit Polyclonal antibody for TNFRSF10D detection. Tested positive for IF, IHC, WB in Human, Mouse, Rat

size :

100 ug

uniprot id :

Q9UBN6

host :

Rabbit

immunogen :

Synthetic peptide corresponding to a portion of human TRAIL-R4.

form :

Liquid. In PBS containing 0.02% sodium azide.

purification :

Peptide affinity purified.

storage :

+4 C

application details :

Immunocytochemistry (10ug/ml) . Western Blot (1:1,000-1:2,000) . Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

applications :

IF, WB

reactivity :

Human, Mouse, Rat

image labels :

Immunocytochemistry analysis of HeLa cells using DcR2 antibody at 10ug/ml. Figure 1. Western blot analysis of TNFRSF10D using anti-TNFRSF10D antibody (A05136). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF10D antigen affinity purified polyclonal antibody (Catalog # A05136) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for TNFRSF10D.

research category :

Apoptosis, Cancer, Cell Biology, Cell Death, Cytokines, Immunology, Innate Immunity, Invasion/Microenvironment, Receptors

last modified :

2/6/19 22:57