Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband™

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband™

catalog :

A04485

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

SKU :

A04485

Product Name :

Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

Application Details :

Western blot, 0.1-0.5µg/ml, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband™ catalog # A04485. Tested in WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

IRF9

Uniprot ID :

Q00978

Immunogen :

E. coli-derived human IRF9 recombinant protein (Position: M1-K110). Human IRF9 shares 87.3% amino acid (aa) sequence identity with mouse IRF9.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Interferon regulatory factor 9

Synonyms :

Interferon regulatory factor 9; IRF-9; IFN-alpha-responsive transcription factor subunit; ISGF3 p48 subunit; Interferon-stimulated gene factor 3 gamma; ISGF-3 gamma; Transcriptional regulator ISGF3 subunit gamma; IRF9; ISGF3G;

Protein Name :

Interferon regulatory factor 9

Molecular Weight :

43696 MW

Protein Function :

Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Subcellular Localization :

Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Background :

Interferon regulatory factor 9 is a protein that in humans is encoded by the IRF9 gene, previously known as ISGF3G. It is mapped to 14q1. IRF9 is a 48 kDa member of the IRF family of proteins. It is widely expressed, and serves as a component of the ISGF3 complex. In addition, IRF9 is an interferon-dependent, positive-acting transcription factor that is cytoplasmically activated, possibly through direct interaction with the interferon receptor. It associates with activated STAT1 and STAT2 to form an ISGF3 complex that is translocated into the nucleus.