Anti-CD11a/ITGAL Antibody Picoband™ | Boster A04466-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CD11a/ITGAL Antibody Picoband™

catalog :

A04466-1

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

A04466-1

Product Name :

Anti-CD11a/ITGAL Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

Flow Cytometry, IHC, ICC, WB

Application Details :

Immunohistochemistry (Frozen Section), 0.5-1µg/ml. Immunocytochemistry, 0.5-1µg/ml Western blot, 0.1-0.5µg/ml.Flow Cytometry, 1-3μg/1x10^6 cells.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-CD11a/ITGAL Antibody Picoband™ catalog # A04466-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

ITGAL

Uniprot ID :

P20701

Immunogen :

E.coli-derived human CD11a recombinant protein (Position: F161-L349). Human CD11a shares 73.7% mino acid (aa) sequence identity with mouse CD11a.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Integrin alpha-L

Synonyms :

Integrin alpha-L; CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain; CD11a; ITGAL; CD11A;

Protein Name :

Integrin alpha-L

Molecular Weight :

128770 MW

Protein Function :

Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

Tissue Specificity :

Leukocytes

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(F) and ICC.

Background :

CD11a, also known as ITGAL or LFA-1, is a human gene which functions in the immune system. It is mapped to 16p11.2. CD11a is involved in cellular adhesion and costimulatory signaling. It is the target of the drug efalizumab. CD11a encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. It is one of the two components, along with CD18, which form lymphocyte function-associated antigen-1.

Research Category :

Cell Adhesion, Cell Type Markers, Cytoskeleton / Ecm, Hematopoietic Progenitors, Immunology, Integrins, Myeloid, Signal Transduction, Stem Cells