Anti-Rac1/CDC42 Antibody | Boster A03252 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Rac1/CDC42 Antibody

catalog :

A03252

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

sku :

A03252

status :

Enabled

name :

Anti-Rac1/CDC42 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

RAC1

price various sizes :

100ug / $299 100ug+Free HRP Secondary BA1054 / $299 100ug+Free Biotin Secondary BA1003 / $299

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

description :

Polyclonal antibody for RAC1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC. Reactive species: Human. RAC1 information: Molecular Weight: 21450 MW; Subcellular Localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Melanosome . Cytoplasm . Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts (By similarity); Tissue Specificity: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

short description :

Anti-Rac1/CDC42 Antibody

size :

100 ug/vial

uniprot id :

P63000

host :

Rabbit

immunogen :

A peptide derived from human Rac1/CDC42. Immunogen sequence location: 38-87

form :

Liquid

purification :

The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

application details :

User need to optimize the dilution ratio for this antibody

applications :

IHC, WB

reactivity :

Human, Mouse, Rat

image labels :

Western blot analysis of lysates from NIH/3T3 cells, treated with EGF 200ng/ml 30', using Rac1/CDC42 Antibody. The lane on the right is blocked with the synthesized peptide. Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Rac1/CDC42 Antibody. The picture on the right is blocked with the synthesized peptide.

research category :

Cancer, G Protein Signaling, Immunology, Innate Immunity, Ras Family, Signal Transduction, Signaling Pathway, Small G Proteins, Tlr Signaling

synonyms :

Ras-related C3 botulinum toxin substrate 1;Cell migration-inducing gene 5 protein;Ras-like protein TC25;p21-Rac1;RAC1;TC25;MIG5;

gene full name :

Ras-related C3 botulinum toxin substrate 1

molecular weight :

21450 MW

protein function :

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.

subcellular localization :

Cell membrane ; Lipid-anchor ; Cytoplasmic side . Melanosome . Cytoplasm . Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts (By similarity).

tissue specificity :

Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

last modified :

2/6/19 22:57