Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband™

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband™

catalog :

A02923

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

SKU :

A02923

Product Name :

Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

IHC, WB

Application Details :

Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat.

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Hydroxysteroid (17-beta) Dehydrogenase 4/HSD17B4 Antibody Picoband™ catalog # A02923. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

HSD17B4

Uniprot ID :

P51659

Immunogen :

E.coli-derived human HSD17B4 recombinant protein (Position: D510-L736). Human HSD17B4 shares 87.7% and 89% amino acid (aa) sequence identity with mouse and rat HSD17B4, respectively.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Peroxisomal multifunctional enzyme type 2

Synonyms :

Peroxisomal multifunctional enzyme type 2; MFE-2; 17-beta-hydroxysteroid dehydrogenase 4; 17-beta-HSD 4; D-bifunctional protein; DBP; Multifunctional protein 2; MPF-2; Short chain dehydrogenase/reductase family 8C member 1; (3R)-hydroxyacyl-CoA dehydrogenase; 1.1.1.n12; Enoyl-CoA hydratase 2; 4.2.1.107; 4.2.1.119; 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase; HSD17B4; EDH17B4, SDR8C1;

Protein Name :

Peroxisomal multifunctional enzyme type 2

Molecular Weight :

79686 MW

Protein Function :

Bifunctional enzyme acting on the peroxisomal beta- oxidation pathway for fatty acids. Catalyzes the formation of 3- ketoacyl-CoA intermediates from both straight-chain and 2-methyl- branched-chain fatty acids.

Subcellular Localization :

Peroxisome.

Tissue Specificity :

Present in many tissues with highest concentrations in liver, heart, prostate and testis.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

Peroxisomal multifunctional enzyme type 2 is a protein that in humans is encoded by the HSD17B4 gene. The protein encoded by this gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathway for fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidation activity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present on chromosome 8. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Research Category :

Cancer, Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Lipids / Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Redox Metabolism, Signal Transduction, Tumor Biomarkers