Anti-CD3 epsilon/Cd3e Antibody Picoband™ | Boster A02765-2 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CD3 epsilon/Cd3e Antibody Picoband™

catalog :

A02765-2

quantity :

100µg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

rat

application :

western blot, ELISA, immunohistochemistry - paraffin section

product information

SKU :

A02765-2

Product Name :

Anti-CD3 epsilon/Cd3e Antibody Picoband™

Size :

100µg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Rat

Application(s) :

ELISA, IHC-P, WB

Application Details :

Western blot, 0.1-0.25µg/ml, Rat. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Rat. Direct ELISA, 0.1-0.5µg/ml, Rat.

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-CD3 epsilon/Cd3e Antibody Picoband™ catalog # A02765-2. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

Cd3e

Uniprot ID :

D4A5M2

Immunogen :

E.coli-derived rat CD3 epsilon/Cd3e recombinant protein (Position: Q21-S108).

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

CD3e molecule

Synonyms :

CD3 antigen, epsilon polypeptide; CD3e molecule; Cd3e; Cd3e_predicted; rCG_58471

Subcellular Localization :

alpha-beta T cell receptor complex; external side of plasma membrane; immunological synapse; cell body; cell-cell junction; dendritic spine; integral component of membrane

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti- Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

CD3e molecule, epsilon also known as CD3E is a polypeptide which in humans is encoded by the CD3E gene which resides on chromosome 11. It is mapped to 11q23.3. The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development.