antibody

Anti-UNG Picoband Antibody

A01672-1

100 ug/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry

Enabled

Anti-UNG Picoband Antibody

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

UNG

Unconjugated / $280 APC / $330 APC-Cy7 / $330 FITC / $330 PE / $330 PE-Cy5 / $330 PE-Cy7 / $330 30ug sample size / $99 100ug / $280 100ug+Free HRP Secondary BA1054 / $280 100ug+Free Biotin Secondary BA1003 / $280

Polyclonal

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

No

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Polyclonal antibody for UNG detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC-P. Reactive species: Human. UNG information: Molecular Weight: 34645 MW; Subcellular Localization: Isoform 1: Mitochondrion; Tissue Specificity: Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.

Rabbit IgG polyclonal antibody for Uracil-DNA glycosylase(UNG) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

100 ug/vial

30ug for $99, contact us for details

P13051

Rabbit

E.coli-derived human UNG recombinant protein (Position: R219-L313). Human UNG shares 92.6% amino acid (aa) sequence identity with mouse UNG.

Lyophilized

Immunogen affinity purified.

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time.Avoid repeated freezing and thawing.

No cross reactivity with other proteins.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry(Paraffin-embedded Section), 0.5-1ug/ml, Human, Mouse, Rat, By Heat . Western blot, 0.1-0.5ug/ml, Human, Mouse, Rat.

IHC, WB

Human, Mouse, Rat

Figure 1. Western blot analysis of UNG using anti- UNG antibody (A01672-1). . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, . Lane 2: rat heart tissue lysates, . Lane 3: mouse skeletal muscle tissue lysates, . Lane 4: 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- UNG antigen affinity purified polyclonal antibody (Catalog # A01672-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UNG at approximately 39KD. The expected band size for UNG is at 35KD. Figure 2. IHC analysis of UNG using anti- UNG antibody (A01672-1). . UNG was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- UNG Antibody (A01672-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 3. IHC analysis of UNG using anti- UNG antibody (A01672-1). . UNG was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- UNG Antibody (A01672-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 4. IHC analysis of UNG using anti- UNG antibody (A01672-1). . UNG was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- UNG Antibody (A01672-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 5. IHC analysis of UNG using anti- UNG antibody (A01672-1). . UNG was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- UNG Antibody (A01672-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Uracil-DNA glycosylase, also known as UNG or UDG, is a human gene though orthologs exist ubiquitously among prokaryotes and eukaryotes and even in some DNA viruses. The first uracil DNA-glycosylase was isolated from Escherichia coli. This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene, which has been confused with this gene, in the literature and some databases.

Dna / Rna, Dna Damage & Repair, Epigenetics And Nuclear Signaling

Uracil-DNA glycosylase ;UDG ;3.2.2.27 ;UNG ;DGU, UNG1 , UNG15 ;

Uracil-DNA glycosylase

34645 MW

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

Isoform 1: Mitochondrion.

Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.

Uracil-DNA glycosylase

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

5/15/19 2:05