Anti-EPHB2 Antibody | Boster A01507 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-EPHB2 Antibody

catalog :

A01507

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

sku :

A01507

status :

Enabled

name :

Anti-EPHB2 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies

gene name :

EPHB2

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

description :

The antibody detects endogenous level of total EPHB2 protein. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

short description :

size :

100 ul

uniprot id :

P29323

host :

Rabbit

immunogen :

Peptide sequence around aa.896~900 (L-S-S-G-I) derived Human from EPHB2.

form :

Liquid

purification :

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

storage :

Store at -20 C

application details :

Western blotting: 1:500~1:1000

applications :

reactivity :

Human, Mouse, Rat

image labels :

Figure 1. Western blot analysis of EPHB2 using anti-EPHB2 antibody (A01507). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHB2 antigen affinity purified polyclonal antibody (Catalog # A01507) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-- IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # -) with Tanon 5200 system. A specific band was detected for EPHB2.

research category :

Angiogenesis, Axonal Guidance Proteins, Cancer, Cardiovascular, Cell Type Marker, Growth And Development, Intracellular Signaling, Kinases, Neurology Process, Neuron Marker, Neuroscience, Neurotransmission, Protein Phosphorylation, Receptor Tyrosine Kinases, Receptors / Channels, Signal Transduction, Tyrosine Kinases

last modified :

2/6/19 22:57