Anti-p70S6K/RPS6KB1 Antibody | Boster A01475 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-p70S6K/RPS6KB1 Antibody

catalog :

A01475

quantity :

100 ug/200 ul

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

sku :

A01475

status :

Enabled

name :

Anti-p70S6K/RPS6KB1 Antibody

category name :

Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

RPS6KB1

price various sizes :

100ug / $299 100ug+Free HRP Secondary BA1060 / $299 100ug+Free Biotin Secondary BA1006 / $299

clonality :

Polyclonal

concentration :

1.0 mg/mL by UV absorbance at 280 nm

conjugate :

contents :

Each vial contains the specific antibody at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% BSA.

description :

Polyclonal antibody for S6K1/RPS6KB1 detection. Host: Goat.Size: 100ug/200ul. Tested applications: IHC. Reactive species: Human. S6K1/RPS6KB1 information: Molecular Weight: 59140 MW; Subcellular Localization: Cell junction, synapse, synaptosome . Mitochondrion outer membrane. Mitochondrion. Colocalizes with URI1 at mitochondrion; Tissue Specificity: Widely expressed.

short description :

Goat Anti-p70S6K/RPS6KB1 Antibody for RPS6KB1 detection tested with WB, IHC in Human, Mouse, Rat, Dog, Cow.

size :

100 ug/200 ul

sample size available :

No sample size available

uniprot id :

P23443

host :

Goat

immunogen :

Synthetic peptide with sequence C-MISKRPEHLRMNL, corresponding to the C Terminus of the protein sequence.

form :

Liquid

purification :

Immunogen Affinity Purified

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.

application details :

Western blot, 0.05-0.1ug/ml. IHC, 2-4ug/ml.

applications :

reactivity :

Human, Mouse, Rat

predicted reactivity :

Bovine, Canine

image labels :

Western blot analysis using rabbit anti-p70 S6 Kinase affinity purified polyclonal antibody (Catalog # A01475) shows detection of a predominant band corresponding to p70S6K in mouse brain extract (right lane) after immunoprecipitation. The left lane shows mock immunoprecipitation.

competitor equivalent skus :

sc 13005 sc 74576 sc 20085 sc 13007 sc 74459 sc 130833 sc 9379 sc 9381 sc 9027 sc 393967 sc 8418 sc 230 sc 514501

research category :

Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Dna / Rna, Dna Damage Response, Epigenetics And Nuclear Signaling, Metabolism, Obesity, Protein Phosphorylation, Ribosome, Ser / Thr Kinases, Signal Transduction, Translation

synonyms :

Ribosomal protein S6 kinase beta-1;S6K-beta-1;S6K1;2.7.11.1;70 kDa ribosomal protein S6 kinase 1;P70S6K1;p70-S6K 1;Ribosomal protein S6 kinase I;Serine/threonine-protein kinase 14A;p70 ribosomal S6 kinase alpha;p70 S6 kinase alpha;p70 S6K-alpha;p70 S6KA;RPS6KB1;STK14A;

gene full name :

Ribosomal protein S6 kinase beta-1

molecular weight :

59140 MW

protein function :

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti- apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1- 2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR.

subcellular localization :

Cell junction, synapse, synaptosome . Mitochondrion outer membrane. Mitochondrion. Colocalizes with URI1 at mitochondrion.

tissue specificity :

Widely expressed.

last modified :

1/28/19 19:39