Anti-beta-Actin ACTB Antibody (HRP) | Boster A01263-HRP product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-beta-Actin ACTB Antibody (HRP)

catalog :

A01263-HRP

quantity :

0.1 mg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human

application :

western blot, ELISA

product information

SKU :

A01263-HRP

Product Name :

Anti-beta-Actin ACTB Antibody (HRP)

Size :

0.1 mg

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat, Rabbit, Chicken, Drosophila

Application(s) :

ELISA, WB

Application Details :

HRP-Beta-Actin antibody can be used for detection of Beta-Actin by Western blot at 0.5 - 1 µ/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-beta-Actin ACTB Antibody (HRP) catalog # A01263-HRP. Tested in ELISA, WB applications. This antibody reacts with Chicken, Drosophila, Human, Mouse, Rabbit, Rat.

Concentration :

1 mg/mL

Gene Name :

ACTB

Uniprot ID :

P60709

Immunogen :

HRP-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.

Form :

Liquid

Contents :

HRP-Beta-Actin antibody is supplied in PBS containing 1% BSA and 0.02% thimerosol.

Purification :

HRP-Beta-Actin antibody is affinity chromatography purified via peptide column.

Isotype :

IgG

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

melanoma cell adhesion molecule

Synonyms :

Cell surface glycoprotein MUC18; Gicerin; Melanoma cell adhesion molecule; Melanoma-associated antigen MUC18; CD146; Mcam; Muc18

Protein Function :

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Subcellular Localization :

Membrane. Single-pass type I membrane protein.

Tissue Specificity :

Detected in melanoma cell lines.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

In humans, the CD146 protein is encoded by the MCAM gene. It is mapped to 9; 9 A5.1. CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM functions as a receptor for laminin alpha 4, a matrix molecule that is broadly expressed within the vascular wall. Accordingly, MCAM is highly expressed by cells that are components of the blood vessel wall, including vascular endothelial cells, smooth muscle cells and pericytes. Its function is still poorly understood, but evidence points to it being part of the endothelial junction associated with the actin cytoskeleton. A member of the Immunoglobulin superfamily, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. It is expressed on chicken embryonic spleen and thymus, activated human T cells, endothelial progenitors such as angioblasts and mesenchymal stem cells, and strongly expressed on blood vessel endothelium and smooth muscle.