Anti-Vinculin VCL Antibody (biotin) | Boster A01207-Biotin product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Vinculin VCL Antibody (biotin)

catalog :

A01207-Biotin

quantity :

0.1 mg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human

application :

western blot, ELISA

product information

SKU :

A01207-Biotin

Product Name :

Anti-Vinculin VCL Antibody (biotin)

Size :

0.1 mg

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat, Rabbit, Chicken

Application(s) :

ELISA, WB

Application Details :

Vinculin antibody can be used for detection of Vinculin by Western blot at 0.5 - 1 µg/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Vinculin VCL Antibody (biotin) catalog # A01207-Biotin. Tested in ELISA, WB applications. This antibody reacts with Chicken, Human, Mouse, Rabbit, Rat.

Concentration :

1 mg/mL

Gene Name :

VCL

Uniprot ID :

P18206

Immunogen :

Biotin-Vinculin antibody was raised against a 23 amino acid peptide near the amino terminus of human Vinculin.

Form :

Liquid

Contents :

Biotin-Vinculin Antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Purification :

Biotin-Vinculin antibody is affinity chromatography purified via peptide column.

Isotype :

IgG

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

mediator complex subunit 4

Synonyms :

Mediator of RNA polymerase II transcription subunit 4; Activator-recruited cofactor 36 kDa component; ARC36; Mediator complex subunit 4; TRAP/SMCC/PC2 subunit p36 subunit; Vitamin D3 receptor-interacting protein complex 36 kDa component; DRIP36; MED4; ARC36; DRIP36; VDRIP; HSPC126

Protein Function :

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Subcellular Localization :

Nucleus.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Background :

Mediator of RNA polymerase II transcription subunit 4 also known as mediator complex subunit 4 (MED4), a component of Mediator or vitamin D3 receptor-interacting protein complex 36 kDa component (DRIP36) is a protein that in humans is encoded by the MED4 gene. It is mapped to 13q14.2. This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.