Anti-MAP2 Antibody | Boster A01201-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-MAP2 Antibody

catalog :

A01201-1

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

product information

sku :

A01201-1

status :

Enabled

name :

Anti-MAP2 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

MAP2

price various sizes :

100ul / $299 100ul+Free HRP Secondary BA1054 / $299 100ul+Free Biotin Secondary BA1003 / $299

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

description :

Rabbit Polyclonal antibody for MAP2 detection. Tested positive for WB, IF in Human, Mouse, Rat.

short description :

Rabbit Polyclonal antibody for MAP2 detection. Tested positive for WB, IF in Human, Mouse, Rat.

size :

100 ul

uniprot id :

P11137

host :

Rabbit

immunogen :

Peptide sequence around aa.1819~1823 (T-A-A-L-A) derived from Human MAP2.

form :

Liquid

purification :

Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.

storage :

At -20 C for one year. After reconstitution, at 4 C for one month. It can also be aliquotted and stored frozen at -20 C for a longer time. Avoid repeated freezing and thawing.

application details :

Western blotting: 1:500~1:1000. Immunofluorescence: 1:100~1:200

applications :

IF, WB

reactivity :

Human, Mouse, Rat

image labels :

Figure 1. Western blot analysis of MAP2 using anti-MAP2 antibody (A01201-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2 antigen affinity purified polyclonal antibody (Catalog # A01201-1) at 0.5 ug/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for MAP2. Immunofluorescence staining of paraffin-embedded Glioma cells of Spinal Cord using MAP2 Antibody #A01201-1.

research category :

Cell Type Marker, Cytoskeleton, Cytoskeleton / Ecm, Microtubules, Neural Stem Cells, Neuron Marker, Neuroscience, Signal Transduction, Stem Cells

last modified :

4/1/19 10:47