antibody

Anti-liver Arginase/ARG1 Antibody Picoband™

A01106

100µg/vial

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry - paraffin section

Anti-liver Arginase/ARG1 Antibody Picoband™

100µg/vial

Polyclonal

Rabbit

Human, Monkey, Mouse, Rat

ELISA, IHC-P, WB

Western blot, 0.25-0.5 μg/ml. Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml. Direct ELISA, 0.1-0.5 μg/ml.

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-liver Arginase/ARG1 Antibody Picoband™ catalog # A01106. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

ARG1

P05089

E.coli-derived human liver Arginase/ARG1 recombinant protein (Position: E25-D183).

Lyophilized

Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Immunogen affinity purified.

No cross reactivity with other proteins.

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

arginase, liver

Arginase-1; Liver-type arginase; Type I arginase; ARG1

Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure.

Cytoplasm. Cytoplasmic granule.

Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes [PMNs]). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

ARG1 (arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes 98% of the arginase activity in liver but is also present in red cells. Using a rat liver ARG1 cDNA clone to probe a human liver cDNA library, Haraguchi et al. (1987) isolated and characterized a cDNA corresponding to the ARG1 gene. The ARG1 gene is mapped on 6q23.2 and the arginase gene contains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was precipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal tract reacted with it. ?Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene.