Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™

catalog :

A01065

quantity :

100μg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, rat

application :

western blot, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section

product information

SKU :

A01065

Product Name :

Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™

Size :

100μg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

Flow Cytometry, IF, IHC-P, ICC, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Human, Mouse, Rat. Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Human, By Heat. Immunocytochemistry/Immunofluorescence, 2µg/ml, Human. Flow Cytometry, 1-3μg/1x10^6 cells, Human.

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Methylmalonyl Coenzyme A mutase Antibody Picoband™ catalog # A01065. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

MUT

Uniprot ID :

P22033

Immunogen :

A synthetic peptide corresponding to a sequence at the N-terminus of human MUT (33-65aa LHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPE), different from the related mouse sequence by one amino acid.

Form :

Lyophilized

Contents :

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Methylmalonyl-CoA mutase, mitochondrial

Synonyms :

Methylmalonyl-CoA mutase, mitochondrial; MCM; 5.4.99.2 ; Methylmalonyl-CoA isomerase; MUT;

Protein Name :

Methylmalonyl-CoA mutase, mitochondrial

Molecular Weight :

83134 MW

Protein Function :

Involved in the degradation of several amino acids, odd- chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.

Subcellular Localization :

Mitochondrion matrix.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Background :

Methylmalonyl-CoA mutase (MUT) is a mitochondrial enzyme that catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA. This gene is mapped to 6p12.3. MUT requires a vitamin B12-derived prosthetic group, adenosylcobalamin (commonly referred to as AdoCbl), to function. And the product of this enzyme, succinyl-CoA, is a key molecule of the TCA cycle.

Research Category :

Apoptosis, Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Cell Cycle Inhibitors, Chromatin Binding Proteins, DNA / RNA, DNA / RNA Binding, DNA Damage & Repair, DNA Damage Response, Epigenetics and Nuclear Signaling, Intracellular, Oncoproteins/Suppressors, P53 Pathway, Transcription, Tumor Suppressors