Anti-IL-21R (ED) Antibody | Boster A00808 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-IL-21R (ED) Antibody

catalog :

A00808

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

product information

sku :

A00808

status :

Enabled

name :

Anti-IL-21R (ED) Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

IL21R

price various sizes :

100ug / $373 100ug+Free HRP Secondary BA1054 / $373 100ug+Free Biotin Secondary BA1003 / $373

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Liquid. In PBS containing 0.02% sodium azide.

description :

Rabbit Polyclonal antibody for IL21R detection. Tested positive for IHC, WB in Human, Mouse, Rat

short description :

Rabbit Polyclonal antibody for IL21R detection. Tested positive for IHC, WB in Human, Mouse, Rat

size :

100 ug

uniprot id :

Q9HBE5

host :

Rabbit

immunogen :

Synthetic peptide corresponding to a portion of human IL-21R precursor.

form :

Liquid. In PBS containing 0.02% sodium azide.

purification :

Peptide affinity purified.

storage :

+4 C

application details :

Immunohistochemistry (10ug/ml) . Western Blot (0.5-1.0ug/ml) . Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

applications :

IHC, WB

reactivity :

Human, Mouse, Rat

image labels :

Figure 2. IHC analysis of IL21R using anti-IL21R antibody (A00808). IL21R was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-IL21R Antibody (A00808) overnight at 4 C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 1. Western blot analysis of IL21R using anti-IL21R antibody (A00808). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL21R antigen affinity purified polyclonal antibody (Catalog # A00808) at 0.5 ug/mL overnight at 4 C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for IL21R.

research category :

Cytokines, Immunology, Innate Immunity, Interleukins

last modified :

2/6/19 22:57