antibody

Anti-Collagen III/COL3A1 Antibody Picoband‚Ñ¢

A00788-3

10 ug sample

99 USD

polyclonal

domestic rabbit

nonconjugated

human, mouse, rat

western blot, ELISA, immunohistochemistry

1

Anti-Collagen III/COL3A1 Antibody Picoband‚Ñ¢

anti-collagen-iii-col3a1-picoband-trade-antibody-a00788-3-boster

Primary Antibodies

COL3A1

315

10 ug sample

99 USD

Full size

315

Full size carrier free

365

Polyclonal

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg NaN 3 .

Boster Bio Anti-Collagen III/COL3A1 Antibody Picoband catalog # A00788-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Boster Bio Anti-Collagen III/COL3A1 Antibody Picoband catalog # A00788-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

100ug/vial

P02461

Rabbit

E. coli-derived human Collagen III/COL3A1 recombinant protein (Position: D1222-E1455).

Lyophilized

Store at -20ÀöC for one year from date of receipt. After reconstitution, at 4ÀöC for one month. It can also be aliquotted and stored frozen at -20ÀöC for six months. Avoid repeated freeze-thaw cycles.

No cross reactivity with other proteins.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Western blot, 0.1-0.5ug/ml. Immunohistochemistry (Paraffin-embedded Section), 0.5-1ug/ml. ELISA, 0.1-0.5ug/ml.

ELISA,IHC,WB

Human,Mouse,Rat

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Figure 1. Western blot analysis of Collagen III using anti-Collagen III antibody (A00788-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate . Lane 2: human U2OS whole cell lysate . Lane 3: human SW620 whole cell lysate . Lane 4: human SGC-7901 whole cell lysate . Lane 5: rat heart tissue lysate . Lane 6: rat skeletal muscle tissue lysate . Lane 7: mouse skeletal muscle tissue lysate. After Electrophoresis proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III antigen affinity purified polyclonal antibody (Catalog # A00788-3) at 0.5 μg/mL overnight at 4°C then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen III at approximately 160KD. The expected band size for Collagen III is at 139KD. Figure 2. IHC analysis of Collagen III using anti-Collagen III antibody (A00788-3). Collagen III was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen III Antibody (A00788-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 3. IHC analysis of Collagen III using anti-Collagen III antibody (A00788-3). Collagen III was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen III Antibody (A00788-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 4. IHC analysis of Collagen III using anti-Collagen III antibody (A00788-3). Collagen III was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen III Antibody (A00788-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 5. IHC analysis of Collagen III using anti-Collagen III antibody (A00788-3). Collagen III was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen III Antibody (A00788-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 6. IHC analysis of Collagen III using anti-Collagen III antibody (A00788-3). Collagen III was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen III Antibody (A00788-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 7. IHC analysis of Collagen III using anti-Collagen III antibody (A00788-3). Collagen III was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6 epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen III Antibody (A00788-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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7/26/18 7:06

3/2/22 2:56

Collagen III/COL3A1 Antibody (A00788-3)

Anti-Collagen III/COL3A1 Antibody Picoband

Boster Bio Anti-Collagen III/COL3A1 Antibody Picoband catalog # A00788-3. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100ug/vial in Lyophilized form antibody.

100

COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene. It is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it is the major ligand of GPR56 in the developing brain. COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.

Collagen,Cytoskeleton / Ecm,Ecm Proteins,Extracellular Matrix,Mesenchymal Stem Cells,Signal Transduction,Stem Cells

Collagen alpha-1 (III) chain; COL3A1

collagen, type III, alpha 1

Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of ADGRG1 in the developing brain and binding to ADGRG1 inhibits neuronal migration and activates the RhoA pathway by coupling ADGRG1 to GNA13 and possibly GNA12.

Extracellular matrix

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

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