Anti-Estrogen Receptor Antibody | Boster A00786 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Estrogen Receptor Antibody

catalog :

A00786

quantity :

50 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

product information

sku :

A00786

status :

Enabled

name :

Anti-Estrogen Receptor Antibody

category name :

Primary Antibodies, Polyclonal Antibodies, IHC ICC IF Antibodies

gene name :

ESR2

price various sizes :

50ug / $563 50ug+Free HRP Secondary BA1054 / $563 50ug+Free Biotin Secondary BA1003 / $563

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.

description :

Rabbit Polyclonal antibody for ESR2 detection. Tested positive for IF, IHC, IHC, WB in Human, Mouse, Rat

short description :

Rabbit Polyclonal antibody for ESR2 detection. Tested positive for IF, IHC, IHC, WB in Human, Mouse, Rat

size :

50 ug

uniprot id :

Q92731

host :

Rabbit

immunogen :

Synthetic peptide corresponding to aa 100-115 (A 100 EPQKSPWCEARSLEH 115 ) of human, mouse and rat ER (estrogen receptor ) .

form :

Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.

purification :

Epitope-affinity purified IgG.

storage :

-20 C

application details :

Gel Shift Assay (1-5ug/ml) . Immunocytochemistry (2-4ug/ml) . Immunohistochemistry (paraffin sections, 1-2ug/ml) . Western Blot (1-2ug/ml) . Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

applications :

IF, IHC

reactivity :

Human, Mouse, Rat

image labels :

Figure 2. IHC analysis of ESR2 using anti-ESR2 antibody (A00786). ESR2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESR2 Antibody (A00786) overnight at 4 C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37 C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. Figure 1: Estrogen receptor beta, pAb (Prod. No. A00786) used in immunofluorescence.

research category :

Cancer, Chip'Ing Antibodies, Diabetes, Domain Families, Endocrine System, Epigenetics And Nuclear Signaling, Metabolism, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Obesity, Signal Transduction, Signaling Pathway, Transcription, Tumor Biomarkers, Zinc Finger

last modified :

2/6/19 22:57