Anti-Bid Antibody Picoband™ | Boster A00730 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-Bid Antibody Picoband™

catalog :

A00730

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

mouse, rat

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

product information

SKU :

A00730

Product Name :

Anti-Bid Antibody Picoband™

Size :

100 ug/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Mouse, Rat

Application(s) :

Flow Cytometry, IHC-P, WB

Application Details :

Western blot, 0.1-0.5µg/ml, Mouse. Immunohistochemistry (Paraffin-embedded Section), 2-5µg/ml, Rat, By Heat. Flow Cytometry, 1-3 μg/1x10^6 cells, Mouse.

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-Bid Antibody Picoband™ catalog # A00730. Tested in Flow Cytometry, IHC-P, WB applications. This antibody reacts with Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

Bid

Uniprot ID :

P70444

Immunogen :

E.coli-derived mouse Bid recombinant protein (Position: M1-D195). Mouse Bid shares 64.6% and 87.2% amino acid (aa) sequence identity with human and rat Bid, respectively.

Form :

Lyophilized

Contents :

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

BH3-interacting domain death agonist

Synonyms :

BH3-interacting domain death agonist; p22 BID; BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID; Bid;

Molecular Weight :

21952 MW

Protein Function :

Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c.

Subcellular Localization :

Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic. p15 BID translocates to mitochondria as an integral membrane protein. p13 and p22 BID are associated with the mitochondrial membrane.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, the human BID gene is mapped to 22q11. It is reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Research Category :

Apoptosis, Apoptotic Markers, Cancer, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Metabolism, Metabolism Processes, Pathways And Processes