Anti-LEF1 Antibody | Boster A00605 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-LEF1 Antibody

catalog :

A00605

quantity :

100 ug/200 ul

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot

product information

sku :

A00605

status :

Enabled

name :

Anti-LEF1 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies

gene name :

LEF1

price various sizes :

100ug / $299 100ug+Free HRP Secondary BA1060 / $299 100ug+Free Biotin Secondary BA1006 / $299

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

Each vial contains the specific antibody at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% BSA.

description :

Polyclonal antibody for LEF 1/LEF1 detection. Host: Goat.Size: 100ug/200ul. Tested applications: WB. Reactive species: Human. LEF 1/LEF1 information: Molecular Weight: 44201 MW; Subcellular Localization: Nucleus . Found in nuclear bodies upon PIASG binding; Tissue Specificity: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.

short description :

Goat Anti-LEF1 Antibody for LEF1 detection tested with WB in Human, Mouse, Rat, Dog.

size :

100 ug/200 ul

sample size available :

No sample size available

uniprot id :

Q9UJU2

host :

Goat

immunogen :

Synthetic peptide with sequence C-QHEQRKEQEPKRPH, corresponding to the internal region of the protein sequence.

form :

Liquid

purification :

Immunogen Affinity Purified

application details :

Western blot, 1-3ug/ml.

applications :

reactivity :

Human, Mouse, Rat

predicted reactivity :

Canine

image labels :

Anti-LEF1 Antibody (1Âµg/ml) staining of MOLT4 lysate (35Âµg protein in RIPA buffer). Detected by chemiluminescence.

competitor equivalent skus :

sc 374522 sc 374412 sc 56722 sc 8591 sc 28687 sc 8592 sc 81470 sc 8591 X sc 28687 X sc 8592 X sc 374522 X sc 374412 X

research category :

Cancer, Developmental Biology, Domain Families, Epigenetics And Nuclear Signaling, Lineage Markers, Lineage Specification, Nuclear, Oncoproteins/Suppressors, Signaling Pathways, Stem Cells, Transcription, Transcription Factors, Tumor Suppressors

synonyms :

Lymphoid enhancer-binding factor 1;LEF-1;T cell-specific transcription factor 1-alpha;TCF1-alpha;LEF1;

gene full name :

Lymphoid enhancer-binding factor 1

molecular weight :

44201 MW

protein function :

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

subcellular localization :

Nucleus . Found in nuclear bodies upon PIASG binding.

tissue specificity :

Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.

last modified :

2/6/19 22:57