Anti-AMCase/CHIA Antibody Picoband™ | Boster A00437-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-AMCase/CHIA Antibody Picoband™

catalog :

A00437-1

quantity :

100 µg/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human

application :

western blot, ELISA, flow cytometry

product information

SKU :

A00437-1

Product Name :

Anti-AMCase/CHIA Antibody Picoband™

Size :

100 µg/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

WB, Flow Cytometry, ELISA

Application Details :

Western blot, 0.5-1 µg/ml, Mouse, Rat. Flow Cytometry, 1-3 μg/1x10^6 cells, Human. Direct ELISA, 0.1-0.5 µg/ml, Human.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-AMCase/CHIA Antibody Picoband™ catalog # A00437-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Gene Name :

CHIA

Uniprot ID :

Q9BZP6

Immunogen :

E.coli-derived human AMCase/CHIA recombinant protein (Position: L228-A476).

Form :

Lyophilized

Contents :

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification :

Immunogen affinity purified.

Storage :

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Reconstitution :

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Background :

BRCA1-associated RING domain protein 1 is a protein that in humans is encoded by the BARD1 gene. This gene encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. This protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interaction is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. This protein may be the target of oncogenic mutations in breast or ovarian cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.