Anti-EGF Antibody Picoband™ | Boster A00378-1 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-EGF Antibody Picoband™

catalog :

A00378-1

quantity :

100 ug/vial

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

rat

application :

western blot, ELISA, immunohistochemistry

product information

SKU :

A00378-1

Product Name :

Anti-EGF Antibody Picoband™

Size :

100 ug/vial

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Rat

Application(s) :

ELISA, IHC, WB

Application Details :

ELISA, 0.1-0.5µg/ml, Rat, Immunohistochemistry (Paraffin-embedded Section), 0.5-1µg/ml, Rat, By Heat. Western blot, 0.1-0.5µg/ml, Rat.

Application Notes :

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-EGF Antibody Picoband™ catalog # A00378-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat.

Concentration :

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Gene Name :

Egf

Uniprot ID :

P07522

Immunogen :

E. coli-derived rat EGF recombinant protein (Position: N974-R1026). Rat EGF shares 69.8% and 77.4% amino acid (aa) sequence identity with human and mouse EGF, respectively.

Form :

Lyophilized

Contents :

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification :

Immunogen affinity purified.

Cross-reactivity :

No cross reactivity with other proteins.

Storage :

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Reconstitution :

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Gene Full Name :

Pro-epidermal growth factor

Synonyms :

Pro-epidermal growth factor; EGF; Epidermal growth factor; Egf;

Molecular Weight :

124126 MW

Protein Function :

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6 (By similarity).

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Background :

EGF is known as epidermal growth factor. This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. Additionally, it acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Research Category :

Cancer, Cell Biology, Cell Cycle, Cell Differentiation, Growth Factors, Growth Factors/Hormones, Signal Transduction