Anti-GAPDH Antibody (HRP) | Boster A00227-HRP product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-GAPDH Antibody (HRP)

catalog :

A00227-HRP

quantity :

0.1 mg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human

application :

western blot, ELISA

product information

SKU :

A00227-HRP

Product Name :

Anti-GAPDH Antibody (HRP)

Size :

0.1 mg

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat, Rabbit, Chicken

Application(s) :

ELISA, WB

Application Details :

HRP-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 µ/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-GAPDH Antibody (HRP) catalog # A00227-HRP. Tested in ELISA, WB applications. This antibody reacts with Chicken, Human, Mouse, Rabbit, Rat.

Concentration :

1 mg/mL

Gene Name :

GAPDH

Uniprot ID :

P04406

Immunogen :

HRP-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.

Form :

Liquid

Contents :

HRP-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% thimerosol.

Purification :

HRP-GAPDH antibody is affinity chromatography purified via peptide column.

Isotype :

IgG

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

malic enzyme 2

Synonyms :

NAD-dependent malic enzyme, mitochondrial; NAD-ME; Malic enzyme 2; ME2

Subcellular Localization :

Mitochondrion matrix.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Background :

NAD-dependent malic enzyme, mitochondrial is a protein that in humans is encoded by the ME2 gene. It is mapped to 18q21.2. This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene.