Anti-GAPDH Antibody (biotin) | Boster A00227-Biotin product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-GAPDH Antibody (biotin)

catalog :

A00227-Biotin

quantity :

0.1 mg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human

application :

western blot, ELISA

product information

SKU :

A00227-Biotin

Product Name :

Anti-GAPDH Antibody (biotin)

Size :

0.1 mg

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat, Rabbit, Chicken

Application(s) :

ELISA, WB

Application Details :

Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 0.5 - 1 µ/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-GAPDH Antibody (biotin) catalog # A00227-Biotin. Tested in ELISA, WB applications. This antibody reacts with Chicken, Human, Mouse, Rabbit, Rat.

Concentration :

1 mg/mL

Gene Name :

GAPDH

Uniprot ID :

P04406

Immunogen :

Biotin-GAPDH antibody was raised against a synthetic peptide containing 16 amino acids near the carboxy terminus of GAPDH.

Form :

Liquid

Contents :

Biotin-GAPDH antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Purification :

Biotin-GAPDH antibody is affinity chromatography purified via peptide column.

Isotype :

IgG

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

minichromosome maintenance complex component 5

Synonyms :

DNA replication licensing factor MCM5; CDC46 homolog; P1-CDC46; MCM5; CDC46

Protein Function :

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Interacts with MCMBP.

Subcellular Localization :

Nucleus. Cytosol.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Background :

DNA replication licensing factor MCM5 is a protein that in humans is encoded by the MCM5 gene. It is mapped to 22q12.3. The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.