Anti-CD86 Antibody | Boster A00220 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-CD86 Antibody

catalog :

A00220

quantity :

0.1 mg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

product information

SKU :

A00220

Product Name :

Anti-CD86 Antibody

Size :

0.1 mg

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

ELISA, WB, IHC, IF

Application Details :

CD86 antibody can be used for Western blot at 1 - 2 µg/mL. Antibody can also be used for Immunohistochemistry at 2 µg/mL. For Immunoflorescence start at 20 µg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-CD86 Antibody catalog # A00220. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.

Concentration :

1 mg/mL

Gene Name :

Cd86

Uniprot ID :

P42082

Immunogen :

CD86 antibody was raised against a peptide corresponding to 17 amino acids near the center of human CD86. The immunogen is located within amino acids 160 - 210 of CD86.

Form :

Liquid

Contents :

CD86 Antibody is supplied in PBS containing 0.02% sodium azide.

Purification :

CD86 Antibody is affinity chromatography purified via peptide column.

Isotype :

IgG

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

methyl-CpG binding domain 4, DNA glycosylase

Synonyms :

Methyl-CpG-binding domain protein 4; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; MBD4; MED1

Protein Function :

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

Subcellular Localization :

Nucleus.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Background :

Methyl-CpG-binding domain protein 4 is a protein that in humans is encoded by the MBD4 gene. It is mapped to 3q21.3. The protein encoded by this gene is a member of a family of nuclear proteins related by the presence of a methyl-CpG binding domain (MBD). These proteins are capable of binding specifically to methylated DNA, and some members can also repress transcription from methylated gene promoters. This protein contains an MBD domain at the N-terminus that functions both in binding to methylated DNA and in protein interactions and a C-terminal mismatch-specific glycosylase domain that is involved in DNA repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.