antibody

Anti-ITCH/AIF4 Antibody

A00195

100 ug/200 ul

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat

Anti-ITCH/AIF4 Antibody

290 USD

Synthetic peptide with sequence C-EIKSHDLKPNGGN, corresponding to the internal region of the protein sequence.

Liquid

Goat

Each vial contains the specific antibody at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% BSA.

Immunogen Affinity Purified

Polyclonal

Goat Anti-ITCH/AIF4 Antibody for ITCH detection tested with WB in Human, Mouse, Rat, Dog.

100 ug/200 ul

Human; Mouse; Rat

WB

ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

E3 ubiquitin-protein ligase Itchy homolog

ITCH

Canine

Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T-helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation (By similarity). Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination.

cell biology proteolysis / ubiquitin proteasome / ubiquitin ubiquitin e3 enzymes hect e3 ligase

Cell membrane. Cytoplasm . Nucleus. Associates with endocytic vesicles. May be recruited to exosomes by NDFIP1.

E3 ubiquitin-protein ligase Itchy homolog;Itch;6.3.2.-;Atrophin-1-interacting protein 4;AIP4;NFE2-associated polypeptide 1;NAPP1;ITCH;

Widely expressed.

Q96J02