antibody

Anti-Toll-like receptor 2 TLR2 Antibody

A00131-1

0.1 mg

polyclonal

domestic rabbit

nonconjugated

polyclonal

western blot, ELISA, immunohistochemistry, immunocytochemistry

Anti-Toll-like receptor 2 TLR2 Antibody

0.1 mg

Polyclonal

Rabbit

Human, Mouse

ELISA, WB, IHC, IF

TLR2 antibody can be used for detection of TLR2 by Western blot at 1 µg/mL. Despite its predicted molecular weight, TLR2 runs at ~100 kDa in SDS-PAGE. This polyclonal antibody can also detect TLR2 by immunohistochemistry at 2 µg/mL. For immunofluorescence start at 10 µg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Boster Bio Anti-Toll-like receptor 2 TLR2 Antibody catalog # A00131-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.

1 mg/mL

TLR2

O60603

TLR2 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TLR2. The immunogen is located within the first 50 amino acids of TLR2.

Liquid

TLR2 Antibody is supplied in PBS containing 0.02% sodium azide.

TLR2 Antibody is Ion exchange chromatography purified.

IgG

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

glycogen synthase kinase 3 alpha

Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions, leading to activate KAT5/TIP60 acetyltransferase activity and promote acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer.

Microtubule. Cytosol. Mitochondrion. Nucleus.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Glycogen synthase kinase-3 alpha is an enzyme that in humans is encoded by the GSK3A gene. It is mapped to 19q13.2. This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.