Anti-BRD4, HUNK1 Antibody | Boster A00123 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-BRD4, HUNK1 Antibody

catalog :

A00123

quantity :

100 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

product information

sku :

A00123

status :

Enabled

name :

Anti-BRD4, HUNK1 Antibody

category name :

Primary Antibodies, Polyclonal Antibodies

gene name :

BRD4

price various sizes :

25ug sample size / $99 100 ug / $299 100 ug+Free HRP Secondary BA1054 / $299 100 ug+Free Biotin Secondary BA1003 / $299

clonality :

Polyclonal

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide.

description :

Polyclonal antibody for BRD4 detection. Host: Rabbit.Size: 100 ug. Tested applications: WB. Reactive species: Human. BRD4 information: Molecular Weight: 152219 MW; Subcellular Localization: Nucleus. Chromosome. Associates with acetylated chromatin. Released from chromatin upon deacetylation of histones that can be triggered by different signals such as activation of the JNK pathway or nocodazole treatment; Tissue Specificity: Ubiquitously expressed.

short description :

Rabbit IgG Polyclonal antibody for BRD4 detection. Tested with WB in Human.

size :

100 ug

sample size available :

25ug for $99, contact us for details

uniprot id :

O60885

host :

Rabbit

immunogen :

A partial length recombinant BRD4 protein (amino acids 140-500).

form :

Liquid

storage :

Store the antibody at 4 C, stable for 6 months. For long-term storage, store at -20 C. Avoid repeated freeze and thaw cycles.

application details :

Western blot analysis, 2-4 ug/ml.

applications :

reactivity :

Human

image labels :

Western blot analysis of BRD4, HUNK1. Anti- BRD4, HUNK1 antibody was used at 2 ug/ml on SKM lysate.

competitor equivalent skus :

sc 27976 sc 48772 sc 27975

research category :

Cancer, Chromatin Binding Proteins, Epigenetics And Nuclear Signaling, Oncoproteins/Suppressors, Transcription, Tumor Suppressors

synonyms :

Bromodomain-containing protein 4;Protein HUNK1;BRD4;HUNK1;

gene full name :

Bromodomain-containing protein 4

molecular weight :

152219 MW

protein function :

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P- TEFb complex and recruiting it to promoters: BRD4 is required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P- TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II. According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters.

subcellular localization :

Nucleus. Chromosome. Associates with acetylated chromatin. Released from chromatin upon deacetylation of histones that can be triggered by different signals such as activation of the JNK pathway or nocodazole treatment.

tissue specificity :

Ubiquitously expressed.

protein name :

Bromodomain-containing protein 4

last modified :

2/6/19 22:57