Anti-PD-L1 CD274 Antibody | Boster A00109-1 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-PD-L1 CD274 Antibody

catalog :

A00109-1

quantity :

0.1 mg

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry

product information

SKU :

A00109-1

Product Name :

Anti-PD-L1 CD274 Antibody

Size :

0.1 mg

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Application(s) :

ELISA, WB, IHC, IF, Flow Cytometry

Application Details :

WB: 1-2 µg/mL; IHC-P: 2.5-5 µg/mL; IF: 20 µg/mL; Flow Cyt: 0.5 µg/mL. Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human and rat samples; Immunofluorescence in human and rat samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Application Notes :

Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Description :

Boster Bio Anti-PD-L1 CD274 Antibody catalog # A00109-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Concentration :

1 mg/mL

Gene Name :

CD274

Uniprot ID :

Q9NZQ7

Immunogen :

Anti-PD-L1 antibody was raised against a peptide corresponding to 17 amino acids near the center of human PD-L1 isoform 1. The immunogen is located within amino acids 60 - 110 of PD-L1.

Form :

Liquid

Contents :

PD-L1 Antibody is supplied in PBS containing 0.02% sodium azide.

Purification :

PD-L1 Antibody is affinity chromatography purified via peptide column.

Isotype :

IgG

Storage :

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Gene Full Name :

basal cell adhesion molecule (Lutheran blood group)

Synonyms :

Basal cell adhesion molecule; Auberger B antigen; B-CAM cell surface glycoprotein; F8/G253 antigen; Lutheran antigen; Lutheran blood group glycoprotein; CD239; BCAM; LU; MSK19

Protein Function :

Laminin alpha-5 receptor. May mediate intracellular signaling.

Subcellular Localization :

Membrane. Single-pass type I membrane protein.

Tissue Specificity :

Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.

Recommended Detection Systems :

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.

Background :

Basal cell adhesion molecule, also known as Lutheran antigen, is a plasma membraneglycoprotein that in humans is encoded by the BCAM gene. It is mapped to 19q13.32. This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu (a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene.