Anti-IL-6 Antibody | Boster A00102-3 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-IL-6 Antibody

catalog :

A00102-3

quantity :

1 mL

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

polyclonal

reactivity :

human

application :

western blot, immunoprecipitation

product information

SKU :

A00102-3

Product Name :

Anti-IL-6 Antibody

Size :

1 mL

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

Application(s) :

IP, WB

Application Details :

Western Blot: 1:500 - 1:2,000. Immunohistochemistry: 1:400 - 1:800. ImmunoPrecipitation: 1:400 - 1:800. Other Dilution: User Optimized

Description :

Boster Bio Anti-IL-6 Antibody catalog # A00102-3. Tested in IP, WB applications. This antibody reacts with Human.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

IL6

Uniprot ID :

P05231

Immunogen :

This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.

Form :

Liquid (sterile filtered)

Contents :

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Purification :

Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts) . In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule. The antiserum is also useful for neutralization of human of IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity. This product has minimal reactivity with mouse IL-6.

Storage :

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Research Category :

Angiogenesis, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment, Metabolism, Neurology Process, Neuroscience, Obesity, Tumor Immunology